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12 Possible Causes for (Titer):Point, Antibody:Dilution, FLUORESCENCE, Factor, Muscle, IN, sarcolemma, time:Serum:Quantitative:IMMUNE

  • Barth Syndrome

    Following washes, slides were incubated in 2 antibodies diluted (1:200) 1 μg/mL 4′,6-diamidino-2-phenylindole (Sigma Aldrich) in PBS 5% BSA for 1 h at room temperature.[doi.org] We showed that MLCL gave the same fluorescence yield per nanomole as CL.[pnas.org] It should also be considered in the following scenarios: boys with idiopathic neutropenia (particularly where this is intermittent and unpredictable); those with unexplained[ncbi.nlm.nih.gov] Studying insulin-like growth factor (IGF)-1 and growth hormone and the inflammatory cytokines interleukin (IL)-6 and tumour necrosis factor (TNF)-alpha by high-sensitivity[journals.lww.com] Muscle AMP deaminase was deficient (5 resp. 17%).[ncbi.nlm.nih.gov] Nutritional cardiomyopathy (including thiamine, selenium, carnitine and vitamin D deficiency) Idiopathic mitochondrial disease Cyclic or idiopathic neutropenia Management[ojrd.biomedcentral.com] In the bottom panels, values indicate mean fluorescence intensity (MFI) of NAO staining.[dx.doi.org] Nitrocellulose membranes were incubated with 1:2500 diluted peroxidase-labeled antibodies (Promega).[dmm.biologists.org]

    Missing: (Titer):Point
  • Dilated Cardiomyopathy

    After rinsing in 4 SSC, they were incubated under coverslips with fluoresceinated avidin (Vector Laboratories) at a 1:500 dilution in 1% BSA/4 SSC for 1 h at 37 C.[hmg.oxfordjournals.org] Co-transfection experiments of wild-type and mutant desmin conjugated to different fluorescence proteins revealed a dominant affect of this mutant on filament assembly.[ncbi.nlm.nih.gov] Its risk factors remain unclear.[ncbi.nlm.nih.gov] […] to a cause of DCM, especially in neonate Elevated acute phase reactants and cardiac enzymes Myocarditis Positive viral titers Viral myocarditis Low serum carnitine Systemic[transplantbuddies.org] In contrast, scaffold-free skeletal muscle-derived cell (SC) sheets attenuated cardiac dysfunction and arrhythmogenicity via paracrine effects.[ncbi.nlm.nih.gov] Muscle biopsy for histopathology and immunohistochemistry for accessing sarcolemma changes.[ncbi.nlm.nih.gov] ., δ-sarcoglycan), the structural complex that links the contractile apparatus to the sarcolemma and extracellular matrix. 50 These mutations may cause defective transmission[nejm.org] Although this predicts an amino-acid substitution and premature termination (p.Thr1260Argfs*8), muscle biopsy dystrophin immunostaining instead indicates that the mutation[ncbi.nlm.nih.gov]

    Missing: IN
  • Felty's Syndrome

    Peroxidase-conjugated anti-human IgM (1:40 dilution) or anti-human IgG (1:40 dilution) was added, and samples were incubated for 2 hours at room temperature.[onlinelibrary.wiley.com] A Fluorescent in situ hybridization (FISH) study is negative for myelodysplasia profile.[elynsgroup.com] Abstract Felty's syndrome (FS), the association of rheumatoid arthritis (RA) and idiopathic neutropenia, remains an unexplained phenomenon.[ncbi.nlm.nih.gov] Granulocyte colony-stimulating factor (G-CSF) is an emerging treatment for chronic neutropenia.[ncbi.nlm.nih.gov] The clues that lead to the diagnosis of RA were high-titer RF and positive anti-CCP. Anti-CCP has very high specificity for RA-96% [ 8 ].[casesjournal.biomedcentral.com] The diagnosis was confirmed by computerized tomography, and the appropriate muscle compartment was surgically drained.[ncbi.nlm.nih.gov] The diagnosis is based on the patient’s history, imaging techniques, fluorescence-activated cell sorting and, in some cases, molecular biological techniques.[thieme-connect.de] Felty's syndrome may be related to decreased production of hematopoietic growth factors.[ncbi.nlm.nih.gov]

    Missing: sarcolemma
  • Epstein Barr Virus

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.[abcam.com] KEYWORDS: Epstein-Barr virus; Hodgkin lymphoma cell line; IL-10; chromosomal integration; fluorescence in situ hybridization[ncbi.nlm.nih.gov] In conclusion, smoking was an important environmental factor, which associated with increased levels of EBNA1-IgA, and Zta-IgA.[ncbi.nlm.nih.gov] He had seizures and temporary focal neurologic deficits pointing toward involvement of cerebellum and brain stem but predominantly neuropsychologic signs and confusion.[ajnr.org] CD68 and factor XIIIa were found in interstitial cells but not smooth-muscle cells.[doi.org] Human coxsackie-adenovirus receptor is colocalized with integrins alpha(v)beta(3) and alpha(v)beta(5) on the cardiomyocyte sarcolemma and upregulated in dilated cardiomyopathy[dx.doi.org] Wild-type virus at a dilution of 1/200 was able to transform peripheral blood leukocytes, whereas recombinant virus was unable to produce transformation foci at any dilution[ncbi.nlm.nih.gov] Human coxsackie-adenovirus receptor is colocalized with integrins α v β 3 and α v β 5 on the cardiomyocyte sarcolemma and upregulated in dilated cardiomyopathy.[dx.doi.org]

    Missing: IN
  • Ebola Virus

    R266; 1:3000 dilution), a mouse anti-GP monoclonal antibody (cl. 226/8.1; 1:2000 dilution), a rabbit anti-VP30 polyclonal antibody (cl. 3; 1:1000 dilution), or a mouse anti-VP24[doi.org] VSV-G envelope gene was replaced with the EBOV glycoprotein (GP) and green fluorescent protein (GFP) genes (rVSV-EBOVgp-GFP).[ncbi.nlm.nih.gov] Additional host factors in the endosomal compartment are probably required for viral membrane fusion; however, despite considerable efforts, these critical host factors have[ncbi.nlm.nih.gov] Geometric mean titers (horizontal lines) are shown for each group and time point.[nejm.org] Symptoms of Ebola A person infected with Ebola virus will typically develop: a high temperature (fever) a headache joint and muscle pain a sore throat severe muscle weakness[nhs.uk] […] arrived to the Morningside Medi Clinic with a diagnosis of rhabdomyolysis (a clinical and biochemical syndrome resulting from an injury which damages the integrity of the sarcolemma[web.stanford.edu] Symptoms include: fever, headache, joint and muscle aches, weakness, diarrhea, vomiting, rashes and abnormal bleeding.[usd.edu] However, how VP35 function is regulated by host cellular factors is poorly understood.[ncbi.nlm.nih.gov]

    Missing: IN
  • Autosomal Dominant Limb-Girdle Muscular Dystrophy Type 1H

    A JANUS liquid handler (Perkin Elmer), which is connected to the incubator, was used to back-dilute the strains to 0.25 OD into plates containing the same medium.[academic.oup.com] neutropenia [ ] Adult intestinal botulism [ ] Adult intestinal colonization botulism [ ] Adult intestinal toxemia botulism [ ] Adult intestinal toxin-mediated botulism [[wikicure.wikia.com] […] containing 1 * Facio-scapulo-humeral muscular dystrophy, type 2 - FSHMD1B Muscular dystrophy with generalized lipodystrophy - (AD) 1.12 Polymerase I and transcript release factor[195.83.227.65] […] appearance of normal muscle and the pathology of individual muscle disease.[books.google.it] This complex, composed of 5 glycoproteins (α-, β-, γ-, δ-, ε-SG), is a member of the dystrophin-associated glycoprotein (DAG) complex localised to the sarcolemma of muscle[bmcneurol.biomedcentral.com] Gass disease Gastric adenocarcinoma and proximal polyposis of the stomach Gastric carcinoma, salivary gland type Gastric duplication cyst of the tongue Gastric intrinsic factor[orpha.net] V And Factor VIII, Combined Deficiency Of, 1 3 Factor V And Factor VIII, Combined Deficiency Of, 2 3 Factor V Deficiency 3 Factor VII Deficiency 3 Factor X Deficiency 3 Factor[preventiongenetics.com] muscle biopsy.[mendelian.co]

    Missing: (Titer):Point
  • Multiple Sclerosis

    Toxin was detected after adding sera diluted 1000-fold in PBST with 3% (w/v) milk and incubated overnight at 4 C.[journals.sagepub.com] Proteins/metabolism Herpesvirus 4, Human/metabolism* Heterozygote Immunity, Humoral Mice Mice, Inbred BALB C Mice, Inbred C57BL Microscopy, Fluorescence Receptors, Antigen[ncbi.nlm.nih.gov] Infectious Complications In Children With Severe Congenital, Autoimmune Or Idiopathic Neutropenia: A Retrospective Study From The Italian Neutropenia Registry.[emedicine.medscape.com] Other proposed risk factors for MS are mentioned only briefly; although we recognize that some of these might be genuine, evidence is usually sparse and unpersuasive.[ncbi.nlm.nih.gov] […] at later time points during convalescence from infection. 41 We therefore suggest that subtle changes in regulatory mechanisms of EBV persistence early in the development[ncbi.nlm.nih.gov] Differences between ratings after 12 weeks of treatment were statistically significant in favour of CE for sections of the MSSS-88 measuring muscle stiffness, muscle spasms[jnnp.bmj.com] Some of these factors also play a role in the MS disease course.[ncbi.nlm.nih.gov] […] area, tick exposure, rash of erythema chronica migrans) Tuberculin skin testing, chest radiography, or QuantiFERON-TB testing (eg, tuberculosis [TB] exposure, endemic area) Fluorescent[emedicine.com]

    Missing: sarcolemma
  • X-Linked Reducing-Body Myopathy 1A

    Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Fhl1 in 10 µg of human brain tissue lysate.[sigmaaldrich.com] (543 nm) to excite TexasRed and To-Pro fluorescence (Carl Zeiss, Jena, Germany).[hmg.oxfordjournals.org] neutropenia) induces a sustained increase in absolute neutrophil counts in peripheral blood and a reduction of infection and related events es El empleo de filgrastim en[es.glosbe.com] Specific risk factors for the development of statin myopathy include higher doses, smaller body frame, liver and kidney disease, diabetes, hypothyroidism, and genetic factors[clevelandclinicmeded.com] Sewry , PhD, FRCPath, Professor of Muscle Pathology, Dubowitz Neuromuscular Centre, Institute of Child Health and Great Ormond Street Hospital, London, UK; Wolfson Centre[books.google.de] Some of these proteins are located in the muscle fiber just inside the sarcolemma (dystrophin); others are embedded in the sarcolemma (sarcoglycans); and others are located[neuropathology-web.org] Luciano Merlini , Paolo Bonaldo , Emanuele Marzetti Frontiers Media SA , 18.01.2016 - 248 Seiten Loss of muscle mass and increased fibrosis characterize both sarcopenia of[books.google.de] This collection of neuromuscular disorders features the differential clinical phenotypes related to each genotype and are representative of the whole spectrum of a genetic muscle[books.google.de]

    Missing: (Titer):Point
  • Alpha-Hemolytic Streptococcus

    On the basis of numerous minor discrepancies between disk diffusion and agar dilution test results for certain antibiotics, it is proposed that the current NCCLS inhibition[karger.com] […] residue) Type-specific proteins Methods Bacitracin sensitivity Rapid identification tests: Based on extraction of Grp A carbohydrate directly from throat swabs Coagglutination Fluorescent[life.umd.edu] In patients with alpha-hemolytic streptococcus SS, levels of interleukin (IL) 1 were undetectable, levels of tumor necrosis factor (TNF) alpha were only slightly elevated,[ncbi.nlm.nih.gov] Rapid antigen-detection tests that can detect GABHS directly from throat swabs are available (ie, for point-of-care use).[merckmanuals.com] The infected person usually feels tired and has a fever , sometimes accompanied by chills, headache , muscle aches, swollen lymph glands, and nausea.[medical-dictionary.thefreedictionary.com] Other GAS antigens appear to cross-react with cardiac sarcolemma membranes. [16] During the course of the host's immune response to the GAS, the host's antigens may, as a[emedicine.medscape.com] […] amplification and sequencing of the streptolysin S structural gene (sagA) and M protein gene (emm) hypervariable region showed the presence of these suspected primary virulence factors[ncbi.nlm.nih.gov] […] detection of recent infection .clots, therefore are responsible for easy spread of the Group A Streptococci Streptokinases: can lyse blood occurs free DNA present in pus, dilution[forums.prohealth.com]

    Missing: IN
  • Anaerobic Streptococcus

    Objective: This study focused on the biofilm formation of S. mutans serotype K when incubated in various dilutions of nicotine.[scholarworks.iupui.edu] Veillonella can be presumptively identified by the red fluorescence of colonies under ultraviolet light. This fluorescence is lost rapidly on exposure to oxygen.[ncbi.nlm.nih.gov] Immunological aspects such as T cell factors and interleukins have been shown to play an important role in the pathogenesis of intra-abdominal sepsis.[books.google.com] Rapid antigen-detection tests that can detect GABHS directly from throat swabs are available (ie, for point-of-care use).[merckmanuals.com] 884 NEUROSIS AND PSYCHIATRIC DISEASES 897 PSYCHOTROPIC DRUGS 906 OPIATES AND SYNTHETIC ANALGESICS 913 DISEASES OF THE STRIATED MUSCLES IN PREGNANCY 932 GENERAL CONSIDERATIONS[books.google.com] […] streptococcal in origin or it may be a host tissue species with antigenic determinants similar to those of streptococcal antigen (cross-reactive epitopes for endocardium, sarcolemma[ncbi.nlm.nih.gov] O. (2000). β-Ketoacyl-acyl carrier protein synthase III (FabH) is a determining factor in branched-chain fatty acid biosynthesis.[link.springer.com] Tenderness, muscle spasm and rebound tenderness do not become prominent until the pain has shifted.[atsu.edu]

    Missing: IN