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1,484 Possible Causes for Method,, Two Hybrid

  • Echinococcus Granulosus

    As shown by yeast two-hybrid and pull-down analysis, EgSmadE displayed a positive binding interaction with EgSmadD.[ncbi.nlm.nih.gov] Wherever this would be necessary for epidemiological purposes, the method can be adjusted according to local requirements.[ncbi.nlm.nih.gov] However, using serological methods such as ELISA, specific antibodies can be determined.[ncbi.nlm.nih.gov]

  • Classical Swine Fever

    The N(pro)-IkappaBalpha interaction was confirmed using yeast two-hybrid analysis and additional co-precipitation assays.[ncbi.nlm.nih.gov] Acceptability, which describes the willingness of persons to contribute, is often analyzed using participatory methods.[ncbi.nlm.nih.gov] Using a yeast two-hybrid screen, we identified poly(C)-binding protein 1 (PCBP1) as a novel interacting partner of the CSFV N(pro) protein and confirmed this by coimmunoprecipitation[ncbi.nlm.nih.gov]

  • Hemorrhagic Fever with Renal Syndrome

    Performances of the two hybrid models were compared with ARIMA model.[ncbi.nlm.nih.gov] This paper utilized the seasonal-trend decomposition (STL) method to analyze the periodicity and seasonality of the HFRS data, and used the exponential smoothing model (ETS[ncbi.nlm.nih.gov] Figure 1: The disintegrating results of HFRS by the STL method.[nature.com]

  • Echinococcus Multilocularis

    Yeast two-hybrid interaction studies demonstrated that EmSmadE can form homodimers, and is capable of heterodimer formation with the previously identified common Smad (Co-Smad[ncbi.nlm.nih.gov] Monitoring Echinococcus multilocularis viability and spread using real-time fluorescent imaging in vivo provides a fast method to evaluate the load of parasite.[ncbi.nlm.nih.gov] We also characterized a second insulin receptor family member, EmIR2, and demonstrated interaction of its ligand binding domain with human insulin in the yeast two-hybrid[ncbi.nlm.nih.gov]

  • Toxoplasma Gondii

    RESULTS: Yeast two-hybrid (Y2H) experiment was used to identify the binding partners of surface antigens of T. gondii by using SAG2 as bait.[ncbi.nlm.nih.gov] More concise methods are still needed to evaluate the possibility of transfusion-transmitted toxoplasmosis from blood donors. T.[ncbi.nlm.nih.gov] KEYWORDS: Host-pathogen interaction; Protein-protein interaction; Tg14–3-3; Toxoplasma gondii; Yeast two-hybrid system[ncbi.nlm.nih.gov]

  • Astrovirus

    KEYWORDS: Caco-2; Human astrovirus; SMART technology; Yeast two-hybrid; cDNA library[ncbi.nlm.nih.gov] Science Inventory You are here: EPA Home » Science Inventory » DEVELOPMENT OF A MOLECULAR METHOD TO IDENTIFY ASTROVIRUS IN WATER.[cfpub.epa.gov] J Virol Methods. 2013 Mar;188(1-2):126-31. doi: 10.1016/j.jviromet.2012.11.040. Epub 2012 Dec 26.[ncbi.nlm.nih.gov]

  • Leber Congenital Amaurosis

    METHODS: The CytoTrapXR yeast two-hybrid system was used to screen a bovine retinal cDNA library.[ncbi.nlm.nih.gov] METHODS: Pupillary light reflexes (PLR) were measured in patients who had received a unilateral subretinal injection in a clinical gene therapy trial.[ncbi.nlm.nih.gov] METHODS: First, we performed direct Sanger sequencing of 7 LCA disease genes in 81 LCA families.[ncbi.nlm.nih.gov]

  • Bile Salt

    Secondly, a membrane yeast two-hybrid (MYTH) assay was used to determine protein interaction partners using a human liver cDNA library.[ncbi.nlm.nih.gov] Tandem mass spectrometry with a lower limit of detection of 0.01 μmol/liter should be the method of choice.[ncbi.nlm.nih.gov] Comprehensive protocols for assessing BSEP inhibition in a 384-well format using radiolabeled and liquid chromatography/mass spectrometry (LC/MS)-based detection methods are[ncbi.nlm.nih.gov]

  • Sorbitol

    We originally identified RASSF6 as a putative interactor of membrane-associated guanylate kinase inverted (MAGI)-1 by the yeast two-hybrid screening.[ncbi.nlm.nih.gov] The method for routine determination of functional hepatic plasma perfusion proved to be rapid, safe and non-invasive in ICU patients.[ncbi.nlm.nih.gov] The method performs satisfactorily measuring the three most common inositol isomers on 25 μL clinical samples of serum, plasma, milk, and/or urine.[ncbi.nlm.nih.gov]

  • Cervical Spondylosis

    The purpose of this study was to compare the clinical and radiological outcomes of two hybrid reconstructive techniques in noncontiguous 3-level cervical spondylosis (2 contiguous[ncbi.nlm.nih.gov] DESIGN: A meta-analysis of the two anterior fusion methods was conducted.[ncbi.nlm.nih.gov] HILT was more effective than the Saunders method in long-term follow-up.[ncbi.nlm.nih.gov]

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