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333 Possible Causes for sepharose

  • Influenza

    This solution was loaded on a DEAE Sepharose column (diameter, 2.5 cm and height, 5.5 cm; Pharmacia), equilibrated with 20 mM Tris-HCl, pH 8, and 50 mM NaCl.[doi.org]

  • Infectious Mononucleosis

    Antibodies were purified by affinity chromatography using protein G Sepharose 4 fast flow resin (GE Healthcare Biosciences Corp.) according to the manufacturer's instructions[dx.doi.org]

  • Tuberculosis

    Hernandez-Pando R, Orozco H, Mancilla R (1995) T-cell lung granulomas induced by sepharose-coupled Mycobacterium tuberculosis protein antigens: immunosuppressive phenomena[springerlink.com]

  • Vicia Faba

    Giza 843) using cation exchange chromatography on an SP-Sepharose column, anion exchange chromatography on Q-Sepharose and Mono Q columns, and finally size exclusion chromatography[ncbi.nlm.nih.gov] It was adsorbed on Affi-gel blue gel and CM-Sepharose. It exerted antifungal activity toward Mycosphaerella arachidicola and Physalospora piricola.[ncbi.nlm.nih.gov] Abstract When human serum is applied to a column of Sepharose-insolubilized lectin from Vicia faba, some serum proteins are bound which can be eluted by means of 0.1 M glucose[ncbi.nlm.nih.gov]

  • Congenital Goiter

    Only minute amounts of Tg-related material was detected by RIA (0.28 and 0.17 mg/g tissue compared to 80-100 mg/g in normal thyroid tissue), by Sepharose 6B chromatography[ncbi.nlm.nih.gov] Gel filtration (CL6B Sepharose gel) showed absence of a normal Tg peak, and SDS agarose gel electrophoresis indicated complete absence of Tg dimer and monomer.[ncbi.nlm.nih.gov]

  • Mycoplasma Penetrans

    The induction of TNF alpha production and enhancement of HIV-1 replication were strongly inhibited by Concanavalin A-Sepharose.[ncbi.nlm.nih.gov] A ca. 65-kDa fibronectin-binding protein of M. penetrans was eluted following Sepharose-fibronectin affinity chromatography.[ncbi.nlm.nih.gov] The 40-kDa nuclease was extracted from M. penetrans cells by Triton X-114 and phase fractionation and was further purified by chromatography on Superdex 75 and chelating Sepharose[ncbi.nlm.nih.gov]

  • IgE Myeloma

    In sera from atopic patients the binding of IgE on Con A Sepharose 4B column was stronger than in normal persons.[ncbi.nlm.nih.gov] […] antiserum to the kappa, IgE protein Bed in three ways: 1) antibodies to IgE PS (anti-PS) were isolated from the goat antiserum by affinity chromatography with PS coupled to Sepharose[ncbi.nlm.nih.gov]

  • Autoimmune Gastritis

    The pig glycoprotein was purified by chromatography on tomato lectin-Sepharose, and five tryptic peptides from the purified glycoprotein were partially sequenced.[ncbi.nlm.nih.gov] Protein A-Sepharose beads preincubated with immunoglobulins of five individual patient (but not control) sera were all found to reduce both the H ,K -ATPase activity and the[ncbi.nlm.nih.gov] A 0.5% Triton X-100 extract of pig gastric mucosal membranes was initially precleared by sequential passage through Protein A-Sepharose 4B and normal human Ig-Sepharose 4B[ncbi.nlm.nih.gov]

  • Autosomal Recessive Juvenile Parkinson Disease

    […] mitochondrial depolarizing agent) for 3hrs. 0.25mg of whole cell lysate was immunoprecipitated for Parkin with 5ul of PARKIN S966C antibody (3rd bleed) coupled to Protein G sepharose[mrcppureagents.dundee.ac.uk]

  • Decamethonium

    They involve the coupling of choline and its ethyl analogue, triethylcholine to activated Sepharose.[ncbi.nlm.nih.gov] Latest findings AChE was purified using Procainamide Sepharose chromatography and eluted using either Procainamide (0.2 M), Decamethonium (0.02 M) or acetylcholine (0.2 M)[drug.report]

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