Vacuolization: Causes & Reasons

Possible Causes for Vacuolization

Combined Oxidative Phosphorylation Deficiency Type 24

[…] hypercalciuric nephrocalcinosis 308990 CLCN7 16p13.3 Osteopetrosis, autosomal dominant 2 166600 Osteopetrosis, autosomal recessive 4 611490 CLDN1 3q28 Ichthyosis, leukocyte vacuoles [institutobernabeu.com]

[…] dominant SQSTM1 5q35.3 Neurodegeneration with ataxia, dystonia, and gaze palsy, childhood-onset 617145 601530 Autosomal recessive SQSTM1 5q35.3 Myopathy, distal, with rimmed vacuoles [mnglabs.com]

Bartter syndrome, type 4, digenic, 602522 (3) CLDN1 G:603718 Bell>cutaneous Ichthyosis, leukocyte vacuoles, alopecia, and sclerosing cholangitis, 607626 (3) CLDN11 G:601326 [usegalaxy.org]

Lipid Cell Tumor of the Ovary

[…] columns Scant stroma may be fibromatous, edematous or myxoid Tumor cells are round / polygonal with spongy to granular, eosinophilic cytoplasm with clear intracytoplasmic vacuoles [pathologyoutlines.com]

The background shows tiny lipid vacuoles mixed with hemorrhagic material. [ijdvl.com]

Usually unilateral, yellow / brown nodules separated by fibrous trabeculae Microscopic (histologic) description Large rounded or polyhedral cells with abundant eosinophilic or vacuolated [pathologyoutlines.com]

Hibernoma

Granular cell tumour : Distinguished by complete absence of lipid cytoplasmic vacuoles. The granular cells are diffusely positive with S-100 protein. [histopathology-india.net]

Intracytoplasmic vacuoles are not seen in these tumours. See smartphone apps to check your skin. [Sponsored content] Related information [dermnetnz.org]

General features: The tumour is divided into lobules by thin septa: There are three cell types: i) Large coarsely vacuolated cells ii) Large finely vacuolated cells with eosinophilic [histopathology-india.net]

Hilar Cell Tumor of the Ovary

Images hosted on PathOut server: Crystals of Reinke Cellular areas separated by anuclear eosinophilic zones Prominent fibrous stroma Fibrinoid replacement Abundant, pale, vacuolated [pathologyoutlines.com]

Some of these cells had a vacuolated clear appearance suggestive of NOS subtype of steroid cell tumor ( Fig. 2 ). [edmcasereports.com]

[…] demonstrated by Masson's trichrome or iron hematoxylin but frequently difficult to find, are required for the diagnosis of "Hilus or Leydig cell tumor" in ovarian tumors with vacuolated [path.upmc.edu]

Spindle Cell Hemangioma

Some these cells contain vacuoles or intracytoplasmic lumina. Bundles of smooth muscle cells are present around the vascular spaces and in the solid areas. [histopathology-india.net]

Microscopic (histologic) description Bland spindle cell proliferations between vascular lumina with extravasated erythrocytes (similar to Kaposi's sarcoma), but also with vacuolated [pathologyoutlines.com]

[…] dilated, thin walled cavernous vascular spaces and solid areas composed predominantly of spindle cells and clusters of epithelioid endothelial cells with intracytoplasmic vacuoles [indianjcancer.com]

Microsporidiosis

This development can occur either in direct contact with the host cell cytoplasm (E. bieneusi) or inside a vacuole called a parasitophorous vacuole (E. intestinalis). [en.wikipedia.org]

This development can occur either in direct contact with the host cell cytoplasm ( E. bieneusi ) or inside a vacuole called a parasitophorous vacuole ( E. intestinalis ). [en.wikipedia.org]

Either free in the cytoplasm or inside a parasitophorous vacuole, microsporidia develop by sporogony to mature spores. [en.wikipedia.org]

Pleomorphic Liposarcoma

vacuole or vacuoles Must be sharply defined Must indent and distort nucleus May be focal or widespread S100 may highlight lipoblasts May contain cytoplasmic eosinophilic [surgpathcriteria.stanford.edu]

We consistently identified numerous lipoblasts as well as micro and macrovesicular fat vacuoles in the background of cellular, pleomorphic sarcomatoid neoplasms. [ncbi.nlm.nih.gov]

Fine needle aspiration cytology smears displayed prominent arborizing vasculature with numerous lipoblasts with well-defined vacuolated cytoplasm, indenting the nuclei. [ncbi.nlm.nih.gov]

Familial Visceral Myopathy

Histologic abnormalities primarily involved smooth muscle and included marked nuclear enlargement and irregularity, interstitial fibrosis, and cytoplasmic vacuolation. [ncbi.nlm.nih.gov]

Although the lesion most closely resembles progressive systemic sclerosis, the degenerating muscle cells and vacuolated appearance of the muscle serve to distinguish familial [ncbi.nlm.nih.gov]

[…] remnants become surrounded by collagen or as muscle cells are destroyed, leaving apparent spaces surrounded by collagen, the longitudinal and circular muscles take on a vacuolated [ncbi.nlm.nih.gov]

Late-Onset Pompe Disease

PAS-positive lymphocyte vacuoles can be used as diagnostic screening test for Pompe disease. [ncbi.nlm.nih.gov]

[…] as intense staining of subsarcolemmal and cytoplasmic vacuoles (PAS stain x200). d Immunolabeling of multiple membrane-bound cytoplasmic vacuoles with dystrophin (Dystrophin [ncbi.nlm.nih.gov]

However, muscle biopsy can also present with less than 1% or no vacuolated fibers11. Werneck LC, Lorenzoni PJ, Kay CS, Scola RH. Muscle biopsy in Pompe disease. [scielo.br]

Distal Myopathy Type 3

The most outstanding morphological features in DMRV are myopathic changes and rimmed vacuole formation. [10] Such vacuoles are seen in various neuropathic and myopathic processes [neurologyindia.com]

Tubulofilamentous inclusion, myeloid bodies, and autophagic vacuoles in the area corresponding to rimmed vacuoles ( d ; bar = 1 μm). [karger.com]

The CK is slightly high and there are no vacuoles in the muscular biopsy. [scielo.br]

Oculo-Pharyngo-Distal Myopathy

Muscular dystrophy changes with rimmed vacuoles were the main histopathological changes. [ncbi.nlm.nih.gov]

Myopathological findings showed rimmed vacuoles with varying degrees of muscular dystrophic changes. [ncbi.nlm.nih.gov]

Vacuolated muscle fibers c. [s-ibm.org]

Sarcotubular Myopathy

The vacuoles are similar to those described in sarcotubular myopathy. TRIM32 sequencing revealed the common c.1459G>A mutation at homozygosity. [ncbi.nlm.nih.gov]

Myriad small spaces appeared in abnormal fibers in the transverse sections, while in longitudinal sections the vacuolation was segmental in distribution. [mayoclinic.pure.elsevier.com]

Most vacuoles are empty, but rare vacuoles contain green and reddish purple material (arrow; C, modified Gomoritrichrome). [documents.tips]

Limb-Girdle Muscular Dystrophy Type 2G

Rimmed vacuoles. [ncbi.nlm.nih.gov]

Frequent rimmed vacuoles were observed. [ncbi.nlm.nih.gov]

Rimmed vacuoles were characterized by empty spaces with a rim of basophilic material (Figure 2 B). [bmcclinpathol.biomedcentral.com]

Hereditary Proximal Myopathy with Early Respiratory Failure

Vacuoles: Few Distal dystrophy + Rimmed vacuoles Dominant DNAJB6 7q36 10 to 50 years Legs: Distal Normal, or Slight ↑ Myopathic Vacuoles HIBM1 Dominant Desmin 2q35 25 to 40 [neuromuscular.wustl.edu]

DMRV; Nonaka myopathy; Rimmed vacuole myopathy; Quadriceps Sparing Myopathy; GNE myopathy ^ "OMIM Entry # 605820 - Nonaka Myopathy". [en.wikipedia.org]

TDP43 immunoreactivity was observed adjacent to rimmed vacuoles and in areas around cytoplasmic bodies. [bmcneurol.biomedcentral.com]

Oculopharyngeal Muscular Dystrophy

In contrast, patients with distal myopathy with rimmed vacuoles revealed many rimmed vacuoles, on average in 20% of the fibers, and their oculopharyngeal muscles were spared [ncbi.nlm.nih.gov]

Figure 4 Rimmed vacuoles at the deltoid muscle biopsy (modified Gomori stain, 350×). [scielo.br]

Innervated cultures had large cytoplasmic vacuoles in a number of muscle fibers. [ncbi.nlm.nih.gov]

Limb-Girdle Muscular Dystrophy Type 1G

[…] membrane in the vacuoles. [doi.org]

Skeletal muscle biopsy shows myopathic changes and prominent rimmed vacuoles. [mendelian.co]

Muscle biopsy shows dystrophic changes with abnormal nuclei, rimmed vacuoles, and filamentous inclusions. {ECO:0000269 PubMed:23667635}. [genecards.org]

Rhabdomyoma of the Myocardium

However, the cytoplasmic vacuoles tend to be central, with myofibrils at the periphery. [cttr.org]

no myofibers, S100+, desmin-, myoglobin- Histiocytoid myopathy: small tumor nodules, finely granular cells, no large vacuoles, no spider cells; a variant of rhabdomyomatosis [pathologyoutlines.com]

- Purkinje cell tumour ( Foamy myocardial transformation) - Hamartoma (no cytoplasmic vacuolation). Pinterest -(drsampurna) [histopathology-india.net]

Danon Disease

The vacuole is a very dangerous organelle. The malfunction of the vacuole can cause disease such as Danon disease. [organelleproblems.weebly.com]

[…] clusters of autophagic vacuoles, indicative of autolysosomes. [ncbi.nlm.nih.gov]

In the patient, the number of vacuoles increased over the 14-year interval between biopsies, suggesting that the number of vacuolated fibers increases with age, and correlates [ncbi.nlm.nih.gov]

Cholesterol Ester Storage Disease

Approximately 20% of lymphocytes demonstrated between 1 and 7 cytoplasmic vacuoles (panels A-C; May–Grünwald–Giemsa stain). [ashpublications.org]

Abdominal radiography demonstrated characteristic bilateral adrenal calcification (panel E, arrows).Lymphocyte vacuolation may be acquired but is a useful morphological feature [imagebank.hematology.org]

Lymphocyte vacuolation may be acquired but is a useful morphological feature to refine the differential diagnosis of abnormal storage cells associated with neurovisceral disease [ashpublications.org]

Hemangioblastoma

Microscopically, the neoplasm was seen to be composed of vascular channels associated with foamy stromal cells, containing clear cytoplasmic vacuoles. [ncbi.nlm.nih.gov]

Tumor was composed of numerous capillaries and stromal cells with cytoplasmic vacuolization. [ncbi.nlm.nih.gov]

[…] hosted on other servers : Vascular tumor with adjacent gliosis Various images H&E, NSE, S100 and CD34 Cytology description Nonfibrillar stromal cells with large nuclei and vacuoles [pathologyoutlines.com]

Inclusion Body Myositis

The coexistence of ring fibres, rimmed vacuoles and inclusion-body myositis-type inclusions in a child with congenital myopathy has not been previously reported. [ncbi.nlm.nih.gov]

Abstract OBJECTIVE: Sporadic inclusion body myositis (sIBM) pathogenesis is unknown; however, rimmed vacuoles (RVs) are a constant feature. [ncbi.nlm.nih.gov]

Vacuolated muscle fibers c. [ncbi.nlm.nih.gov]

Sertoli Cell Tumor of the Testis

Neoplastic cells focally contained vacuoles of various sizes in the cytoplasm. Cystic formation and cord formation in the hyalinized stroma was also observed. [ncbi.nlm.nih.gov]

Fine needle aspiration (FNA) cytologic smears showed large polygonal tumor cells with abundant finely granular or vacuolated eosinophilic cytoplasm and eccentric nuclei with [ncbi.nlm.nih.gov]

The tumor cells were of medium size and had pale cytoplasm, which sometimes contained large lipid vacuoles; the round nuclei varied from small and dark to large and vesicular [ncbi.nlm.nih.gov]

Lipogranuloma

Ultrastructural study showed intracytoplasmic fat vacuoles of varied size in histiocytes and multinuclear giant cells. [ncbi.nlm.nih.gov]

sclerotic stroma and occasional foreign body giant cells Numerous, variably sized vacuolated spaces Oil Red O stain Positive stains Lipid stains: Oil Red O for frozen tissue [pathologyoutlines.com]

[…] present, lack epithelial lining but may contain giant cells Also T lymphocyte infiltrate ( Pathol Int 2003;53:121 ) Microscopic (histologic) images AFIP images Characteristic vacuoles [pathologyoutlines.com]

Benign Interstitial Cell Tumor of the Testis

Synonyms interstitial cell tumour LM cytoplasmic vacuolization, cytoplasm -- clear to eosinophilic, +/- Reinke crystals (cylindrical crystalloid -- eosinophilic cytoplasmic [librepathology.org]

Microscopically, hexagonally shaped cells with granular, eosinophilic cytoplasm containing lipid vacuoles are seen. [health.am]

Image C Image D Histology: Large, round or polygonal cells with abundant pink or vacuolated cytoplasm and well-defined cell borders (image B), (image C), & (image D). [auanet.org]

Rhinoscleroma

Deep in the dermis, vacuolated Mikulicz cells surrounded by lymphocytes and plasma cells were found. [ncbi.nlm.nih.gov]

A Warthin-Starry stain demonstrating rod-shaped bacilli within vacuolated macrophages (Mikulicz cells) is classic for rhinoscleroma. [f1000research.com]

Histologically, it is characterized by the vacuolated foamy macrophages presence, with bacilli known as Mikulicz cells (see Figure 1); the determination factors that lead [scielo.sa.cr]

Microglandular Adenosis

[…] uniform, rounded, open glands with eosinophilic secretions, irregularly distributed in fibrous or adipose tissue Glands lined by single layer of cuboidal / flat cells with vacuolated [pathologyoutlines.com]

[…] cribriforming With atypia, complex architecture With atypia, S100 With atypia, collagen IV Cytology description Sparse cellularity, monotonous population of medium sized cells with vacuolated [pathologyoutlines.com]

The glands are lined by a single layer of cells with distinctly vacuolated or granular cytoplasm. [ncbi.nlm.nih.gov]

Acquired Amyloid Myopathy

Vacuoles rimmed with basophilic granules. Inclusion body myositis IBM. Vacuole filled with granules. Modified Gomori trichrome stain. Inclusion body myositis IBM. [neuropathology-web.org]

[…] connective tissue Storage material PAS Glycogen ; Carbohydrate Alcian blue Mucopolysaccharide Sudan black B Lipid Oil red O Lipid Other Congo red Amyloid ; Inflammation; Vacuoles [grants.hhp.uh.edu]

A muscle biopsy obtained from a weak muscle reveals rimmed vacuoles on routine histochemical studies. [nature.com]

Sideroblastic Anemia

Important clues to the diagnosis include cytoplasmic vacuolization of erythroid and myeloid precursors along with ringed sideroblasts on bone marrow evaluation. [ashpublications.org]

Characteristic vacuolization of a hematopoietic precursor in the bone marrow. [emedicine.com]

Bone marrow revealed hypercellularity with cytoplasmic vacuolization of myeloid and erythroid precursors (panels A-B; original magnification ×1000, Wright-Giemsa stain). [ashpublications.org]

Silicone Granuloma

Wick Images hosted on other servers: Material consistent with silicone Liquid silicone droplets Various images Subcapsular sinus diffusely expanded Vacuoles which contain [pathologyoutlines.com]

Silicon was detected in the vacuoles of macrophages and multiple nucleated giant cells by X-ray microanalysis. [ncbi.nlm.nih.gov]

Tissue reaction to silicone liquid and gel was characterized by numerous round to oval empty cystic vacuoles, mild to moderate fat necrosis, foreign body reaction, a variable [jpatholtm.org]

Polyglucosan Body Myopathy Type 2

DMRV; Nonaka myopathy; Rimmed vacuole myopathy; Quadriceps Sparing Myopathy; GNE myopathy ^ "OMIM Entry # 605820 - Nonaka Myopathy". [en.wikipedia.org]

The vacuoles also stain intensely to acid phosphates, confirming that the vacuoles filled with glycogen are secondary lysosomes. [annalsofneurosciences.org]