First-strand cDNA was synthesized as follows: 1 μg of DNase-treated total RNA together with 0.5 μg of oligo(dT) 12–18 (Pharmacia, Uppsala, Sweden) in a total volume of 11 μl were heated to 70 C for 10 min and then chilled on ice. [cancerres.aacrjournals.org]

Characteristics include red or dark patches on the skin; burning, itching, swelling, hardening, and tightening of the skin; yellow spots on the whites of the eyes; joint stiffness with trouble moving or straightening the arms, hands, legs, or feet; pain [emedicine.medscape.com]

A 29-year-old man presented with scintillation scotoma. MR imaging demonstrated a diffuse lesion in right parahippocampal gyrus. He underwent a biopsy, and the diagnosis was diffuse astrocytoma. [ncbi.nlm.nih.gov]

[…] sixth decade and peak in the seventh to eight decades. [2, 3] Clinical Features Clinical symptoms of diffuse astrocytomas are the result of brain irritation (eg, seizures), increased intracranial mass (eg, headaches), or brain invasion (eg, hemiparesis, dysphasia [emedicine.medscape.com]

The possibility that tumors develop novel aberrations, with relevant biological properties, is often neglected. [molecularcytogenetics.biomedcentral.com]

Re-evaluation of archival FCD cases with diverse markers revealed that reactive microglia significantly proliferate in the white matter lesions. [ncbi.nlm.nih.gov]